Some aspects of germination induction in Phycomyces blakesleeanus by an ammonium-acetate pretreatment
✍ Scribed by Delvaux, E.
- Book ID
- 104762418
- Publisher
- Springer-Verlag
- Year
- 1973
- Weight
- 644 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0003-9276
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✦ Synopsis
Dormancy in ~he sporangiospores of Phycomyces blakesleeanus can be broken by a short pretreatmen~ (10 rain at 30~ with NI-Ia-acetate. The effect is partly reversible.
Acetate activation is accompanied by a transient rise in trehalase activity, which causes a sharp decrease in the reserve substance trehalose, followed by an accumulation of glucose in the surrounding medium. At the same time pyruvate, acetaldehyde, ethyl alcohol and lactate can be detected in the culture medium.
CO 2 production by respiration of externally supplied glucose is predominant in dormant and in germinating spores. During acetate treatment most of the CO s produced, is supplied by the turnover of endogenous material.
High activity of the pentese-phosphate (P-P) pathway occurs in dormant spores, as measured by the CJC1 ratio. Adding acetate results in a sudden rise in the glycolyric Krebs cycle (EMP) pathway. Afterwards, the P-P pathway also increases and it predominates again during the initial phases of germination.