Some aspects of fractionation of DNA on an IR-120 Al3+ column : VII. Effect of the tissue and source variation on the chromatographic profiles of DNA

✍ Scribed by R.M. Kothari

• Publisher: Elsevier Science
• Year: 1972
• Tongue: English
• Weight: 968 KB
• Volume: 64
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)92951-0

✦ Synopsis

The effect of variations in the tissue and source on' the chromatographic profiles of DNA using an IR-120' Ala* column was studied. For this purpose, DNA preparations were isolated from buffalo liver, kidney, brain and lung (tissue variation, same source) and from the livers of buffalo, ,humans, albino rat, pigeon and frog (source variation, same tissue). The pure and native preparation, in each case, were'chromatographed and their profiles were studied. Ruffalo liver DNA was completely' retainable, while DNA preparations from the other tissues and sources were either partially retainable or non-retainable. They became completely retainable;, however, after equilibration with 0.001 M Mg2+, indicating a' major influence of Mga+. The elu'tion profiles' of all Mgs+-equilibrated DNAs were similar, Mg2+ possibly stabilized a specific conformation of DNA necessary for retention on the IR-120 Als+ column. Mg2+ could be replaced by Mn 2+, Zns+, Nis+, Ca2+ and IIg2+ in conferring the retainability on buffalo kidney DNA which was originally retainable to an extent of only 7,o %. Arginine also had an equivalent effect.