Some Aspects of Chemical and Pharmacological Studies of Acorus calamus Linn.**Received June 15, 1959, from the Department of Pharmacology, M. G. M. Medical College, Indore, India.
✍ Scribed by Bose, B.C. ;Vijayvargiya, R. ;Saifi, A.Q. ;Sharma, S.K.
- Publisher
- Elsevier
- Year
- 1960
- Weight
- 261 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0095-9553
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✦ Synopsis
ethyl acetate; shake mechanically for five minutes. Aspirate off the aqueous phase, add about 5 Gm. of filter cel to the solvent phase, and filter the latter into a 100-ml. graduated cylinder or volumetric flask. When 100 ml. of filtrate has been collected transfer it to a 250-ml. beaker and evaporate to dryness.
Now proceed with the standard operations of hydrolysis, etc.. finally extracting the aromatic aldehyde into 25 ml. of chloroform. Of this, transfer 5 ml. to a 40-ml. glass-stoppered centrifuge tube and proceed with the p-nitrophenylhydrazine reaction in the usual way. Read the absorbances a t 570 and 700 ma, taking the net value (570-700) as the true reading. The results are evaluated against raceophenidol standards (0,100, and 200 mcg. in 75 ml. of acetone) carried through the entire procedure. The sample finally analyzed represents 0.6 Gm. of feed.
S i x samples of feed were analyzed as described above; two were blanks and four were mixed to contain 25 Gm./ton of raceophenidol. The results were as follows: blank feed, -0.25, -0.25 Gm./ton; medicated feed, 22.0, 23.5, 24.9, 23.5 Gm./ton; mean net recovery, 23.75 f 1.2 Gm./ton, or 95 f 5y0 of claim.
It may be noted that the readings on the blank feed were less than the reagent blank, therefore, the negative values for these samples.
Conclusions
Dextrosulphenidol and raceophenidol may be determined colorirnetrically in a wide variety of materials as the p-nitrophenylhydrazone of pmethylsulfonylbenzaldehyde. The recovery from the materials studied ranges from 70 to 100%; blank values on such materials as tissues and eggs do not usually exceed 0.2 p. p. m. The least amount of these compounds which can be determined satisfactorily is 1 mcg. a t the last step of the analysis. This requires the presence of at least 2-3 mcg. in the initial sample.