✦ LIBER ✦

Somatic transgenesis using retroviral vectors in the chicken embryo

✍ Scribed by Yasuo Ishii; David E. Reese; Takashi Mikawa

Publisher: John Wiley and Sons
Year: 2004
Tongue: English
Weight: 472 KB
Volume: 229
Category: Article
ISSN: 1058-8388
DOI: 10.1002/dvdy.10484

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✦ Synopsis

Abstract

The avian embryo is an excellent model system for experimental studies because of its accessibility and ease of microsurgical manipulations. While the complete chicken genome sequence will soon be determined, a comprehensive germ cell transmission‐based genetic approach is not available for this animal model. Several techniques of somatic cell transgenesis have been developed in the lpast decade. Of these, the retroviral shuttle vector system provides both (1) stable integration of exogenous genes into the host cell genome, and (2) constant expression levels in a target cell population over the course of development. This review summarizes retroviral vectors available for the avian model and outlines the uses of retroviral‐mediated gene transfer for cell lineage analysis as well as functional studies of genes and proteins in the chick embryo. Developmental Dynamics 229:630–642, 2004. © 2004 Wiley‐Liss, Inc.

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