Somatic embryogenesis in protoplast derived calli of cultivated jute,Corchorus capsularisL.

✍ Scribed by Thakurdas Saha; S. K. Sen

• Book ID: 104675007
• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 891 KB
• Volume: 10
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00232386

✦ Synopsis

Protoplasts were isolated from cotyledon, hypocotyl and mesophyll cells of Corchorus capsularis L., a major fibre crop, by one step enzyme digestion method. They were further cultured successfully on modified KM-8p (Kao and Michayluk, 1975) medium to form microcalli. The required cultural conditions could be used to achieve 34% to 78% plating efficiency, depending upon the source of protoplasts. Hypocotyl protoplasts gave the highest plating efficiency. On transfer to regeneration medium, somatic embryos developed at high frequency. The present success is a significant step forward in the development of meaningful plant cell culture methods for application in jute.