Somatic embryogenesis in cotton (Gossypium) I. Effects of source of explant and hormone regime

✍ Scribed by Norma L. Trolinder; J. R. Goodin

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 586 KB
• Volume: 12
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00043105

✦ Synopsis

Optimal media for induction of somatic embryogenesis from mature and immature tissues of G. hirsutum L. cv Coker 312 were determined. Explants of three-day-old seedlings form somatic embryos in 100% of cultures when treated with 0.1 mg/l 2,4-dichlorophenoxyacetic acid plus 0.5 mg/1 kinetin. Mature tissues are more recalcitrant than immature tissues and formed somatic embryos on a limited number of hormone treatments. Stem tissue is most readily induced to form somatic embryos by 2 mg/l napthaleneacetic acid plus 0.1 mg/l kinetin, whereas leaf tissue formed embryos best when treated with 0.1 mg/12,4-dichlorophenoxyacetic acid plus 1.0 mg/l (2-isopentyl)-adenine, or 1.0 mg/1 napthaleneacetic acid plus 0.5 mg/1 (2-isopentyl)-adenine.