Somatic embryogenesis from mature embryo-derived leaflets of peanut (Arachis Hypogaea L.)

✍ Scribed by K. Chengalrayan; Sangeeta S. Sathaye; Sulekha Hazra

• Book ID: 104675788
• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 738 KB
• Volume: 13
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00234515

✦ Synopsis

Embryogenic masses were obtained from immature leaves of peanut (Arachis hypogaea L.) cultured on a medium containing 20 mg/l 2,4-D. Somatic embryos developed from these masses following transfer to a medium containing 3 mg/l 2,4-D. The embryo morphology was quite variable. Following transfer to hormone-free medium, these embryos germinated. Shoot elongation was obtained in 25% of the embryos following transfer to a medium supplemented with 0.5 mg/l each of BAP and Kn. The plants grown in vitro by this method survived in sand:soil mixture and were grown to maturity.