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Somatic embryogenesis and fertile green plant regeneration from suspension cell-derived protoplasts of rye (Secale cerealeL.)

✍ Scribed by R. Ma; Y.-D. Guo; S. Pulli


Publisher
Springer
Year
2003
Tongue
English
Weight
379 KB
Volume
22
Category
Article
ISSN
0721-7714

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Cell suspension cultures, which retained embryogenic potential for almost 2 years, were established from young, expanding, juvenile leaves of a mature Thevetia peruviana L. tree. Calli were obtained by culturing young leaf discs on MS medium supplemented with 2 mg/L 2, 4-dichlorophenoxyacetic acid (