Somaclonal variation in watercress for resistance to crook root disease

✍ Scribed by Dawn L. Arnold; Andrew Flegmann; John M. Clarkson

• Book ID: 104675487
• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 313 KB
• Volume: 14
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00233641

✦ Synopsis

Watercress (Rorippa nasturtium-aquaticum) shoots were regenerated from callus initiated from petioles on a range of media. The best coefficient of regeneration was obtained when callus was induced on a medium containing 0.25 mgl(-1) thidiazuron and 0.05 mgl{si-1} 2,4-D, followed by regeneration on 0.25 mgl(-1) thidiazuron. Plants were then screened for resistance to crook root disease of watercress in a laboratory screening test which revealed significant somaclonal variation between the controls and some of the somaclones.