Solution synthesis of 14C-labeled thymopoietin32–36 using catalytic transfer hydrogenation as the final deprotection step
✍ Scribed by Foe-Siong Tjoeng; George A. Heavner
- Book ID
- 102369747
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- French
- Weight
- 305 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
A ^14^C‐labeled pentapeptide, [^14^C]Arg‐Lys‐Asp‐Val‐Tyr (6), corresponding to the active fragment 32–36 of thymopoietin II has been synthesized by a solution method with a 44 percent radiochemical yield. ^14^C‐labeled Z‐Arg(HCl)‐OH was coupled to the protected tetrapeptide Lys(Z)‐Asp(OBzl)‐Val‐Tyr(Bzl)‐OBzl using dicyclohexylcarbodiimide and 1‐hydroxybenzotriazole to give the protected pentapeptide 5 with a specific activity of 340 mCi/mmol. Catalytic transfer hydrogenation was used to remove the protecting groups and the free peptide was purified by gel filtration and high performance liquid chromatography.