Isolation and partial characterization o
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David D. Dean; Aristotle J. Domnas
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Article
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1983
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Springer
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English
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Lagenidium giganteum Couch produced an extracellular collagenolytic enzyme when grown on peptone-yeast extract-glucose (PYG) broth. The enzyme was purified to apparent homogeneity by chromatography on soybean trypsin inhibitor Sepharose 4B and DEAE cellulose. The purified enzyme hydrolyzed Azocoll,