SOLUBLE DI- AND AMINOPEPTIDASES IN ESCHERICHIA COLI K-12 Dispensible Enzymes
β Scribed by HERMSDORF, CAROL L. ;SIMMONDS, SOFIA ;SAUNDERS, ALDA
- Book ID
- 115097476
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 2009
- Tongue
- English
- Weight
- 443 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0367-8377
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The pepN gene has been cloned into the multicopy plasmid pBR322. The restriction map of the insert was established and the gene was localized. By comparison with the restriction map of the plasmid pJP30 bearing the ompF region, it has been possible to order the ompF, asnS, and pepN genes. The ompF a
Two routes of one-carbon biosynthesis have been described in Escherichia coli K12. One is from serine via the serine hydroxymethyltransferase (SHMT) reaction, and the other is from glycine via the glycine cleavage (GCV) enzyme system. To isolate mutants deficient in the GCV pathway, we used a select