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Solubilization of sphingomyelin by triton X-100 effect of the method of mixing and the concentrations of detergent and lipid

✍ Scribed by Shaul Yedgar; Rachel Hertz; Shimon Gatt

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
671 KB
Volume
13
Category
Article
ISSN
0009-3084

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✦ Synopsis

Mixed dispersions of sphingomyelin and Triton X-100 were prepared by two procedures. In method A, aqueous dispersions of sphingomyelin were mixed with aqueous solutions of Triton X-100. In method B, solutions of sphingomyelin and Triton X-100 in organic solvent were mixed, the solvent was evaporated and the dry residue was dispersed in buffer. Measurement of turbidities, electron microscopy and sedimentation of the mixed dispersions suggested the following: Below the critical miceUar concentration of Triton X-100, the sphingomyelin is present as liposomes which sediment in the ultracentrifuge. Above the CMC, mixed micelles of sphingomyelin and Triton form. Method B resulted in aggregates of sphingomyelin which contain Triton X-t00 even below its critical micellar concentration and which are smaller than those obtained by method A.

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