Solubilization of membrane proteins for two-dimensional gel electrophoresis: identification of sarcoplasmic reticulum membrane proteins
β Scribed by Gopal J Babu; Debra Wheeler; Oscar Alzate; Muthu Periasamy
- Book ID
- 108047619
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 182 KB
- Volume
- 325
- Category
- Article
- ISSN
- 0003-2697
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## Abstract Electrophoretic techniques, and especially twoβdimensional gel electrophoresis (2βDE), have provided an indispensable set of tools for the separation of complex protein mixtures as well as for the identification of proteinβprotein interactions. Nevertheless, after its introduction more
Two-dimensional electrophoresis of proteins is often precluded due to the lack of solubilization of cell membrane extracts in an aqueous medium. Various additives and detergents have been used to circumvent the problem, but their efficacy may not be satisfactory. In this study, the removal of lipidi