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Solubilization of calfskin collagen in citrate buffer with the use of automated equipment

โœ Scribed by Leo D. Kahn; Lee P. Witnauer

Publisher
John Wiley and Sons
Year
1969
Tongue
English
Weight
741 KB
Volume
13
Category
Article
ISSN
0021-8995

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โœฆ Synopsis

An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubiliiation of 6.95% of a calfskin corium as monomeric collagen in citrate buffer a t pH 3.44 and ionic strength of 0.44 and a temperature of 8ยฐC. It is shown that this is the limit of solubiliiation for undenatured collagen in this buffer.

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