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Solubilization and purification of β-hydroxy-β-methylglutaryl coenzyme A reductase from rat liver

✍ Scribed by Kawachi, Takashi; Rudney, Harry


Book ID
126222909
Publisher
American Chemical Society
Year
1970
Tongue
English
Weight
620 KB
Volume
9
Category
Article
ISSN
0006-2960

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Solubilization of 3-hydroxy-3-methylglut
✍ Zafarul H. Beg; John A. Stonik; H.Bryan Brewer Jr. 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 312 KB

A simple, efficient, freeze-thaw procedure for the solubilization of liver 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase has been developed. Microsomes of chicken or rat liver were prepared by homogenization in buffer containing 100 mM sucrose, 50 mM KCI, 40 mM KH,PO,, 30 mM EDTA. and 2