Solubilization and partial purification of lymphocyte specific antigens in the chicken

✍ Scribed by D. Malchow; W. Droege; J. L. Strominger

• Publisher: John Wiley and Sons
• Year: 1972
• Tongue: English
• Weight: 614 KB
• Volume: 2
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830020107

✦ Synopsis

Solubilization and partial purification of lymphocyte specific antigens in the chicken*

Four different lymphocyte antigens were solubilized from chickens homozygous at the B locus. The antigens were identified by immunoelectrophoresis with rabbit anti-lymphocyt e sera.

1. A bursa-specific cell surface antigen was extracted with 3 M potassium chloride and partially purified. The antigen was excluded from Sephadex G-200 gels, was heat labile and was a potent immunogen. T h e antigen could be detected on all bursa cells by immunofluorescence but not on thymus, spleen or blood lymphocytes. The findings d o not exclude, however, the possibility that a small number of lymphocytes (e.g. plasma cells) possess the antigen.

2. A thymus-specific antigen was obtained by papain treatment of thymus cells. It migrated cathodically in immunoelectrophoresis.

A surface antigen which was specific for lymphocytes from both central lymphoid organs (thymus and bursa) was solubilized by pestle homogenization.

4. A fourth lymphocyte surface antigen was present o n lymphocytes from blood, spleen, bursa and thymus. It was best solubilized by 3 M potassium chloride extraction and did not migrate under t h e conditions of immunoelectrophoresis.

Studies did not reveal a stable antigenic marker with specificity for thymus or bursa cells and their progeny in the peripheral lymphocyte pool.