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Solubility study of albumin solders for laser tissue-welding

✍ Scribed by Lauto, A.; Poppas, D. P.; Murrell, G. A. C.

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
155 KB
Volume
23
Category
Article
ISSN
0196-8092

No coin nor oath required. For personal study only.

✦ Synopsis

Background/Objective: Current albumin solders for tissuewelding are soluble in physiological fluids, prior to laser irradiation. These solders are therefore subjected to mechanical alterations, which can weaken the solder-tissue repair. In this study, an albumin solder (laser activated) was developed with low solubility and with the ability to retain (partially) its mechanical characteristics in saline solution. Study Design/Materials and Methods: Gauged protein samples of solder were immersed into 0.5 ml saline solution for fixed intervals of time. The solder samples contained four Bovine Serum Albumin (BSA) concentrations: 56%, 66%, 70%, and 75% (by weight). A Bradford protein assay measured the BSA solubility of the solders. The 70% and 75% BSA solders were also used to weld in vitro Wistar rat intestine sections with a diode laser ( = 810 nm, power = 270 mW). Results: The solubility of the 75% BSA solder was significantly decreased with respect to the other solders (Anova, P < 0.05). This solder also showed comparable weld strength (13 gm) to the 70% BSA solder.

Conclusion:

The 75% BSA solder strongly reduced the albumin solubility in saline solution, without affecting its tissue-welding properties.

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