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Solubility of collagen from normal and scleroderma fibroblasts in culture

✍ Scribed by R.I. Bashey; S. Halpern; R.E. Stephens; J.S. Perlish; R. Fleischmajer


Book ID
119405860
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
247 KB
Volume
62
Category
Article
ISSN
0006-291X

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Fibroblasts cultured from affected skin areas of five patients with cutaneous scleroderma were found to produce increased amounts of collagen when compared with nonaffected control cells. Total RNA was isolated from the cultures and analyzed for its level of proal(1)collagen mRNA by hybridization of