Solid–liquid equilibria for solutions of binary globular-protein mixtures
✍ Scribed by Toshiaki Hino; John M. Prausnitz
- Publisher
- American Institute of Chemical Engineers
- Year
- 1999
- Tongue
- English
- Weight
- 162 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0001-1541
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✦ Synopsis
A theory of the ®an der Waals form is used to establish solid ᎐ fluid phase diagrams for aqueous solutions containing two kinds of globular proteins. Theory is based on a one-fluid model that uses a composition-dependent hard-sphere diameter and a composition-dependent sol®ent-mediated effecti®e temperature to represent protein solutions. The hard-sphere reference equation of state is based on the model of Young that correlates computer simulations of fluid ᎐ solid coexistence cur®es for binary hard-sphere mixtures. The attracti®e perturbation term uses the in®erse-power potential with ®ariable exponent n. Based on the correlation of George and Wilson and Rosenbaum et al., the optimum range of effecti®e temperature is determined for protein crystallization. For binary protein systems, as the size difference increases, the mutual solubility of unlike proteins declines sharply in the precipitated solid phase. The theoretical results obtained here may be useful for design of protein-separation processes by crystallization.