## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a βFull Textβ option. The original article is trackable v
Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays
β Scribed by John E. Butler
- Publisher
- John Wiley and Sons
- Year
- 2004
- Weight
- 17 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0931-7597
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The orientation of an immobilized antigen is important for recognition by, e.g., an antibody. When noncovalent passive adsorption is used for immobilization, the number of ways that the antigen can attach to the surface is numerous and control of how the antigen orientates on the surface is limited.
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Solid-phase ELISAs for the determination of EGF receptor (EGF-R) and pp60c-src tyrosine protein kinase activity are described. The methods were developed and optimized using purified recombinant EGF-R intracellular domain (ICD) and pp60c-src tyrosine protein kinases. A standardized assay that utiliz