Solid-Supported Biomolecules on Modified Silica Surfaces—A Tool for Fast Physicochemical Characterization and High-Throughput Screening

✍ Scribed by A. Loidl-Stahlhofen; J. Schmitt; J. Nöller; T. Hartmann; H. Brodowsky; W. Schmitt; J. Keldenich

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 177 KB
• Volume: 13
• Category: Article
• ISSN: 0935-9648
• DOI: 10.1002/1521-4095(200112)13:23<1829::aid-adma1829>3.0.co;2-3

✦ Synopsis

Biofunctionalization for a wide variety of applications can be achieved by coating silica surfaces with biomolecules such as lipids or proteins. However, specific surface optimization of the inorganic SiO 2 is necessary to achieve biocompatible surfaces. Surface shielded porous silica beads can be non-covalently coated with a single lipid bilayer. The lipids retain their fluidity in this handy solid-supported system, perfectly mimicking the soft-surface properties of cellular membranes. A supramolecular architecture can also be used for functional immobilization of membrane proteins: An artificial cytosolic compartment can be created with the aid of polymers; coating by lipid membranes and integration of membrane proteins results in a solid-supported biofunctional cellular surface. Another surface modification enables a direct immobilization of human serum albumin (HSA) molecules onto silica surfaces. The HSA on this otherwise passivated surface provides a convenient material for the investigation of unspecific protein binding of pharmaceuticals on a high-throughput scale.