Solid-State NMR Determination of 13 Cα Chemical Shift Anisotropies for the Identification of Protein Secondary Structure
✍ Scribed by Hong, Mei
- Book ID
- 126167190
- Publisher
- American Chemical Society
- Year
- 2000
- Tongue
- English
- Weight
- 178 KB
- Volume
- 122
- Category
- Article
- ISSN
- 0002-7863
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📜 SIMILAR VOLUMES
A simple technique for identifying protein secondary structures through the analysis of backbone 13C chemical shifts is described. It is based on the Chemical-Shift Index [Wishart et al. (1992) Biochemistry, 31, 1647-1651] which was originally developed for the analysis of 1H(alpha) chemical shifts.
A solid state NMR technique for the determination of peptide backbone conformations at specific sites in unoriented samples under magic angle spinning (MAS) is described and demonstrated on a doubly labeled polycrystalline sample of the tripeptide AlaGlyGly and a sextuply labeled lyophilized sample