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Solid-phase synthesis of a 5′-terminal TMG-capped trinucleotide block of U1 snRNA

✍ Scribed by Michinori Kadokura; Takeshi Wada; Kohji Seio; Tomohisa Moriguchi; Jochen Huber; Reinhard Lührmann; Mitsuo Sekine

Publisher: Elsevier Science
Year: 2001
Tongue: French
Weight: 102 KB
Volume: 42
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(01)01941-4

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✦ Synopsis

A 5%-terminal 2,2,7-trimethylguanosine-capped trinucleotide block (m 3 2,2,7 G 5% pppAmpUmpA) was successfully synthesized on a highly cross-linked polystyrene resin by a new method for introduction of the first nucleoside onto the resin and a newly developed pyrophosphorylating agent having a benzotriazolyloxy substitutent as the leaving group.

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