Solid-phase synthesis and purification of a set of uniformly 13C, 15N labelled de novo designed membrane fusogenic peptides

✍ Scribed by Prashant R. Agrawal; Mathias W. Hofmann; Nico J. Meeuwenoord; Dmitri V. Filippov; Holger Stalz; Frans Hulsbergen; Dieter Langosch; Hermen S. Overkleeft; Huub De Groot

• Book ID: 105360611
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 154 KB
• Volume: 13
• Category: Article
• ISSN: 1075-2617
• DOI: 10.1002/psc.786

✦ Synopsis

Abstract

The transmembrane segments of soluble N‐ethylmaleimide‐sensitive factor (SNARE) proteins or viral envelope proteins drive membrane fusion, which suggests that simple synthetic biology constructs for fusion exist and can be evaluated. We describe the high‐yield synthesis of a set of de novo designed fusogenic peptides for use in functional investigations, which are highly enriched in ^13^C and ^15^N using three equivalents of labelled amino acids and optimized reaction conditions minimizing aggregation. The biomimetic peptides have a high purity >90% and show reproducible and fusogenic activity that correlates well with the intended functional design characteristics, from strongly fusogenic to almost non‐fusogenic. Copyright © 2006 European Peptide Society and John Wiley & Sons, Ltd.