Solid-Phase Radioreceptor Assay for Paralytic Shellfish Toxins

✍ Scribed by M.R. Vieytes; A.G. Cabado; A. Alfonso; M.C. Louzao; A.M. Botana; L.M. Botana

• Book ID: 102966335
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 549 KB
• Volume: 211
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1237

✦ Synopsis

Sodium channels obtained from rat brain membrane preparations were coated onto microtiter plates and used to develop a direct solid-phase binding assay. The tritiated sodium channel blocker saxitoxin (\left(\left[{ }^{3} \mathrm{H}\right]-\right.) saxitoxin; STX) was used to detect toxins in paralytic shellfish poisoning (PSP) by measuring the competitive displacement of other toxins. With this assay the amount of STX and tetrotoxin needed to displace (50 %) of bound (\left[{ }^{3} \mathrm{H}\right] \mathrm{STX}) was 1.7 and (1.76 \mathrm{ng} / \mathrm{ml}) for buffer samples, respectively. In the direct solid-phase binding assays, the PSP toxins were effectively bound to the rat brain membranes. The (\mathrm{IC}_{50}) of this assay for different PSP toxin solutions obtained from mussels contaminated in red tides ranged from 0.03 to (0.30 \mathrm{ng} / \mathrm{ml}). Therefore, this assay represents a potentially useful method for the detection of toxin-contaminated mussels. ç 1993 Academic Press, Inc.