Solid-Phase Peptide Synthesis

✍ Scribed by Gregg B. Fields (Eds.)

• Publisher: Academic Press
• Year: 1997
• Tongue: English
• Leaves: 821
• Series: Methods in Enzymology 289
• Edition: 1
• Category: Library

✦ Synopsis

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volumehas been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 275 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchers in all fields of life sciences.

Key Features
Solid-phase peptide synthesis
Applications of peptides for structural and biological studies
* Characterization of synthetic peptides

✦ Table of Contents

Content:
Contributors to volume 289
Pages ix-xi

Preface
Page xiii
Gregg B. Fields

Volumes in series
Pages xv-xxxi

[1] Concept and early development of solid-phase peptide synthesis Original Research Article
Pages 3-13
Bruce Merrifield

[2] Rapid in situ neutralization protocols for Boc and Fmoc solid-phase chemistries Original Research Article
Pages 14-29
Paul Alewood, Dianne Alewood, Les Miranda, Stephen Love, Wim Meutermans, David Wilson

[3] Cleavage methods following Boc-based solid-phase peptide synthesis Original Research Article
Pages 29-44
John M. Stewart

[4] Standard Fmoc protocols Original Research Article
Pages 44-67
Donald A. Wellings, Eric Atherton

[5] Trifluoroacetic acid cleavage and deprotection of resin-bound peptides following synthesis by Fmoc chemistry Original Research Article
Pages 67-83
Cynthia A. Guy, Gregg B. Fields

[6] Properties of solid supports Original Research Article
Pages 83-104
Morten Meldal

[7] Coupling reagents and activation Original Research Article
Pages 104-126
Fernando Albericio, Louis A. Carpino

[8] Handles for solid-phase peptide synthesis Original Research Article
Pages 126-174
Michael F. Songster, George Barany

[9] Solid-phase synthesis of cyclic homodetic peptides Original Research Article
Pages 175-198
Christopher Blackburn, Steven A. Kates

[10] Disulfide bond formation in peptides Original Research Article
Pages 198-221
Ioana Annis, Balazs Hargittai, George Barany

[11] Direct synthesis of glycosylated amino acids from carbohydrate peracetates and Fmoc amino acids: Solid-phase synthesis of biomedicinally interesting glycopeptides Original Research Article
Pages 221-245
Jan Kihlberg, Mikael Elofsson, Lourdes A. Salvador

[12] Synthesis of phosphopeptides using modern chemical approaches Original Research Article
Pages 245-266
John William Perich

[13] Protein synthesis by chemical ligation of unprotected peptides in aqueous solution Original Research Article
Pages 266-298
Tom W. Muir, Philip E. Dawson, Stephen B.H. Kent

[14] Synthesis of proteins by subtiligase Original Research Article
Pages 298-313
Andrew C. Braisted, J. Kevin Judice, James A. Wells

[15] Convergent solid-phase peptide synthesis Original Research Article
Pages 313-336
Fernando Albericio, Paul Lloyd-Williams, Ernest Giralt

[16] Synthetic peptide libraries Original Research Article
Pages 336-392
Michal Lebl, Viktor KrchnГЎk

[17] Edman sequencing as tool for characterization of synthetic peptides Original Research Article
Pages 395-419
Gregory A. Grant, Mark W. Crankshaw, John Gorka

[18] Amino acid analysis Original Research Article
Pages 419-426
Alan J. Smith

[19] Analysis of synthetic peptides by high-performance liquid chromatography Original Research Article
Pages 426-469
Colin T. Mant, Leslie H. Kondejewski, Paul J. Cachia, Oscar D. Monera, Robert S. Hodges

[20] Capillary electrophoresis Original Research Article
Pages 469-478
Agustin Sanchez, Alan J. Smith

[21] Fast atom bombardment mass spectrometry of synthetic peptides Original Research Article
Pages 478-499
Е ГЎrka BeranovГЎ-Giorgianni, Dominic M. Desiderio

[22] Analysis of peptide synthesis products by electrospray ionization mass spectrometry Original Research Article
Pages 499-519
Daniel J. Burdick, John T. Stults

[23] Laser desorption mass spectrometry Original Research Article
Pages 520-542
William T. Moore

[24] Protein signature analysis: A practical new approach for studying structure-activity relationships in peptides and proteins Original Research Article
Pages 545-564
Tom W. Muir, Philip E. Dawson, Michael C. Fitzgerald, Stephen B.H. Kent

[25] In vitro incorporation of synthetic peptides into cells Original Research Article
Pages 564-571
Janelle L. Lauer, Gregg B. Fields

[26] Construction of biologically active protein molecular architecture using self-assembling peptide-amphiphiles Original Research Article
Pages 571-582,IN1-IN2,583-587
Ying-Ching Yu, Teika Pakalns, Yoav Dori, James B. McCarthy, Matthew Tirrell, Gregg B. Fields

[27] Chemical synthesis and nuclear magnetic resonance characterization of partially folded proteins Original Research Article
Pages 587-611
Elisar Barbar, Christopher M. Gross, Clare Woodward, George Barany

[28] Multiple antigen peptide system Original Research Article
Pages 612-637
James P. Tam, Jane C. Spetzler

[29] Relaxin Original Research Article
Pages 637-646
John D. Wade, Geoffrey W. Tregear

[30] Solution nuclear magnetic resonance characterization of peptide folding Original Research Article
Pages 646-672
Kevin H. Mayo

[31] Solid-state nuclear magnetic resonance characterization of gramicidin channel structure Original Research Article
Pages 672-696,IN3-IN4
T.A. Cross

[32] Six-year study of peptide synthesis Original Research Article
Pages 697-717
Ruth Hogue Angeletti, Lynda F. Bonewald, Gregg B. Fields

Author index
Pages 719-761

Subject index
Pages 763-780