Solid-phase microextraction gas chromatography-mass spectrometry determination of monoterpenes in honey

✍ Scribed by Rosa M. Peña; Julia Barciela; Carlos Herrero; Sagrario García-Martín

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 107 KB
• Volume: 27
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200301705

✦ Synopsis

Abstract

Solid‐phase microextraction (SPME) using a 100 μm poly(dimethylsiloxane) (PDMS) fiber, followed by gas chromatography (GC‐MS) determination, has been applied to the analysis of some monoterpenoids in honey. The extraction was performed by direct immersion of the fiber using a sampling period of 15 min with constant magnetic stirring (1100 rpm) and an extraction temperature of 20°C. A 7 mL sample volume of an aqueous solution of honey with 25% of NaCl was placed in 15 mL glass vial fitted with screw cap and PTFE/silicone septum. Desorption was performed directly in the gas chromatograph injector port during 5 min at 250°C using the splitless mode. The method is sensitive with detection limits between 11 and 25 μg L^–1^, precise with coefficients of variation in the range 1.28 and 3.71%, and linear over more than one order of magnitude. The related conditions were used for honey sample analyses with recoveries between 71.8 and 90.9%. SPME remains an attractive alternative technique due to its rapidity and because it is a solvent free extraction method.