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Solid-phase genotoxicity assay for organic compounds in soil

✍ Scribed by Renee R. Alexander; Namhyun Chung; Martin Alexander

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
66 KB
Volume
18
Category
Article
ISSN
0730-7268

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✦ Synopsis

A genotoxicity assay was developed for samples from environments in which toxic organic compounds are largely sorbed. The assay entails measurement of the rate of mutation of a strain of Pseudomonas putida to rifampicin resistance. The ratio of induced to spontaneous mutants was a function of the concentration of a test mutagen in soil. In studies of the utility of the assay in samples amended with 2-aminofluorene as a test mutagen, the ratio of induced to spontaneous mutants declined with time. The decline paralleled the disappearance of extractable 2-aminofluorene from the soil. The ratio of induced to spontaneous mutants also fell in four other soils with dissimilar properties. We suggest that this solid-phase assay is more appropriate for the estimation of genotoxicants sorbed in soil than assays involving extractants or suspensions of soil or sediment samples.

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