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Sol–gel immobilization of Alcalase from Bacillus licheniformis for application in the synthesis of C-terminal peptide amides

✍ Scribed by Livia N. Corîci; August E. Frissen; Dirk-Jan van Zoelen; Ivo F. Eggen; Francisc Peter; Corneliu M. Davidescu; Carmen G. Boeriu


Book ID
113804910
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
537 KB
Volume
73
Category
Article
ISSN
1381-1177

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A novel method useful for selective isolation of the C-terminal peptide from a tryptic digestion mixture of a protein has been developed by taking advantage of a unique property of anhydrotrypsin, which has a strong specific affinity for the peptides containing arginine or lysine at their C-termini.