✦ LIBER ✦
Soldium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins: Comparison of dansylation, Coomassie Blue R-250 and silver detection methods
✍ Scribed by Dr. Josie A. Beeley; Frances Newman; Paul H. R. Wilson; Ian C. Shimmin
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 272 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
Dental
Detection of human parotid salivary proteins by dansylation and UV-transillumination after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) has been compared with Coomassie Blue R-250 and silver staining procedures. Dansylation gives superior results in terms of both resolution and sensitivity, especially with basic proline-rich proteins (PRPs).