Sodium valproate stimulates potassium and chloride urinary excretion in rats: gender differences
✍ Scribed by Eitautė Jakutiene; Jurgita Grikiniene; Arunas Vaitkevicius; Marina Tschaika; Janina Didziapetriene; Donatas Stakisaitis
- Publisher
- BioMed Central
- Year
- 2007
- Tongue
- English
- Weight
- 686 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1471-2210
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✦ Synopsis
Background
The diuretic effect of valproates and its relation to urinary potassium (K^+^) and chloride (Cl^-^) excretion have not yet been investigated, so the aim of this study was to evaluate the influence of a single dose of sodium valproate (NaVPA) on 24-h urinary K^+^ and Cl^-^ excretion in young adult Wistar rats of both genders. For measurement of K^+^ in urine, the same animals and samples as in our earlier publication were used (Pharmacology 2005 Nov, 75:111–115). The authors propose a new approach to the pathophysiological mechanisms of NaVPA effect on K^+^ and Cl^-^ metabolism.
Twenty six Wistar rats were examined after a single intragastric administration of 300 mg/kg NaVPA (13 NaVPA-male and 13 NaVPA-female), 28 control intact Wistar rats (14 males and 14 females) were studied as a control group. The 24-h urinary K^+^, Cl^-^, creatinine and pH levels were measured.
Results
Total 24-h diuresis and 24-h diuresis per 100 g of body weight were found to be significantly higher in NaVPA-rats of both genders than in rats of the control group (p < 0.05). The data showed NaVPA to enhance 24-h K^+^ excretion in NaVPA-males and NaVPA-females with significant gender-related differences: 24-h K^+^ excretion in NaVPA-male rats was significantly higher than in control males (p = 0.003) and NaVPA-female rats (p < 0.001). Regarding the 24-h K^+^ excretion, NaVPA-female rats did not show a statistically significant difference versus females of the control group (p > 0.05). 24-h urinary K^+^ excretion per 100 g of body weight in NaVPA-male rats was significantly higher than in control males (p = 0.025). NaVPA enhanced Cl^-^ urinary excretion: 24-h Cl^-^ urinary excretion, 24-h urinary Cl^-^ excretion per 100 g of body weight and the Cl^-^/creatinine ratio were significantly higher in NaVPA-male and NaVPA-female rats than in gender-matched controls (p < 0.05). 24-h chloriduretic response to NaVPA in male rats was significantly higher than in female rats (p < 0.05).
Conclusion
NaVPA causes kaliuretic and chloriduretic effects with gender-related differences in rats. Further investigations are necessary to elucidate the mechanism of such pharmacological effects of NaVPA.