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Sodium Ion Transport in Rat Hearts during Cold Ischemic Storage: 23Na and 31P NMR Study

✍ Scribed by Nadir Askenasy; Antonio Vivi; Maria Tassini; Gil Navon

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
920 KB
Volume
28
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

The success of heart transplantation is limited by the negative correlation between the length of the cold ischemic storage period and the quality of functional recovery. We use ^23^Na, ^31^P NMR spectroscopy, and hemodynamic parameters to describe temperature‐dependent changes in sodium influx and the concentration of phosphorus high‐energy compounds during different storage periods. Perfusion with Krebs—Henseleit solutions containing Dy(TTHA)^3‐^ permitted discrimination of intra‐ and extracellular sodium during cold ischemic storage. The ^23^Na NMR visibilities under the acquisition and processing parameters used in our experiments were 40 ± 4% for the intracellular compartment and 97 ± 11% for the extracellular compartment. At 4°C, the intracellular Na^+^ accumulation exceeded that observed at 15 and 22°C. The ATP and PCr depletion rates were much lower at 4°C and the left ventricular contractility was higher after longer periods of storage, as the storage temperature decreased. The intracellular Na^+^ concentration cannot serve as a marker for the postischemic recovery probability. The relative activity of the Na/K ATPase pumps is not correlated with the preservation success. However, intracellular sodium ion accumulation is a major factor in the time lag of the reperfusion recovery.© 1992 Academic Press,Inc.

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