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Sodium influxes in renal epithelial LLC-PK1/CI4 cells monitored by 23Na NMR

✍ Scribed by A. W. H. Jans; R. Willem; E. R. Kellenbach; R. K. H. Kinne

Book ID: 102528618
Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 424 KB
Volume: 7
Category: Article
ISSN: 0740-3194
DOI: 10.1002/mrm.1910070306

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✦ Synopsis

23Na NMR experiments with a suspension of the renal epithelial cell line, LLC-PK1/Cl4, in the presence of the shift reagent dysprosium polyphosphate showed that the intracellular sodium was only NMR visible for 64 +/- 4%. Intracellular sodium content was found to be 30.6 +/- 1.2 mM (25 degrees C). Examination of the sodium influx during recovery from intracellular acidification showed that sodium is transported not only by Na+/H+ exchange but also by sodium-D-glucose cotransport with a stoichiometry of 1:1.

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