Sodium-dependent glutamate uptake as an activator of oxidative metabolism in primary astrocyte cultures from newborn rat
✍ Scribed by Gun Eriksson; Anders Peterson; Kerstin Iverfeldt; Erik Walum
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 497 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0894-1491
No coin nor oath required. For personal study only.
✦ Synopsis
In the present report we describe the effect of glutamate on respiratory activity in primary cultures of astrocytes, derived from cerebral cortex of newborn rat.
Glutamate (100 FM) caused an increased oxygen consumption. This effect could not be inhibited by antagonists to the NMDA or AMPAkainate receptors. Neither trans-ACPD (an agonist to the metabotropic glutamate receptor) nor the Krebs cycle intermediate a-ketoglutarate had any effect on the respiratory rate. An uncontrolled influx of Na+, caused by gramicidin, could mimic the glutamate effect on respiratory activity. In addition, the glutamate effect was abolished by addition of ouabain or replacement of Na+ by Li+ in the perfusion buffer.
We conclude that the co-transport of Naf , in the Naf -dependent high-affinity glutamate uptake system, mediated the glutamate-induced increase in oxygen consumption through an increased activity of Na+/K+-ATPases. o 1995 Wiley-Liss, Inc.