𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Sodium arsenite suppresses human papillomavirus-16 E6 gene and enhances apoptosis in E6-transfected human lymphoblastoid cells

✍ Scribed by Ruey-Hwang Chou; Haimei Huang

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
209 KB
Volume
84
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The p53 tumor suppressor pathway is disrupted by human papillomavirus (HPV) in most cervical cancer cells. The E6 proteins, which could mediate p53 degradation, are related to cellular immortalization, transformation, and tumor formation. In order to study the E6 abrogated p53 function in stress, we transfected HPV‐16 E6 gene to TK6 cells in this study. Here we showed that HPV‐16 E6 mRNA levels decreased in a dose dependent manner after sodium arsenite (SA) treatment, but not after X‐irradiation. P53, p21, and MDM2 were induced in E6‐transfected TK6 cells, as well as in parental TK6 cells after arsenite treatment. But the above proteins were only induced in TK6 cells after X‐irradiation. It indicated that arsenite, but not X‐ray, could suppress the transcription of E6 gene and therefore activate the p53 tumor suppressor pathway in TK6‐E6 cells. After arsenite treatment, TK6‐E6 cells showed more sub‐G1 apoptosis, activated caspase‐3/CPP32 fragment, DNA ladder, and less viability than parental TK6 cells, indicating that arsenite enhanced apoptosis in E6‐transfected TK6 cells. In contrast, after X‐irradiation, TK6‐E6 cells showed less sub‐G1 apoptosis and higher viability than parental TK6 cells. Thus, it would be another possible strategy to promote arsenite as another potential candidate for the therapeutic purpose in HPV‐positive cancer cells. J. Cell. Biochem. 84: 615–624, 2002. © 2001 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Inhibition of apoptosis in human larynge
Inhibition of apoptosis in human laryngeal cancer cells by E6 and E7 oncoproteins of human papillomavirus 16
✍ Han Ching Liu; George G. Chen; Alexander C. Vlantis; Gary M. Tse; Anthony T.C. C 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 749 KB

## Abstract The carcinogenesis of human papillomaviruses type 16 (HPV‐16) is mainly due to its two oncoproteins, E6 and E7. Their carcinogenic features in term of their relationship with Bcl‐2 family are still unclear. We thus aimed to analyze the expression of Bcl‐2 family members, Bcl‐2, Bax, and

Human papillomavirus type 16 E6-enhanced
Human papillomavirus type 16 E6-enhanced susceptibility to apoptosis induced by TNF in A2780 human ovarian cancer cell line
✍ Faina Vikhanskaya; Carla Falugi; Piera Valente; Patrizia Russo 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 538 KB

## Abstract In our study, we show that expression of HPV‐16 E6 sensitizes TNF‐induced cytotoxicity of human ovarian cancer cell line A2780. This effect is not related to a different number of TNF receptors present on cell membrane. The major induction of massive apoptosis induced by TNF is not p53‐

Selective suppression of monocyte chemoa
Selective suppression of monocyte chemoattractant protein-1 expression by human papillomavirus E6 and E7 oncoproteins in human cervical epithelial and epidermal cells
✍ Kerstin Kleine-Lowinski; James G. Rheinwald; Raina N. Fichorova; Deborah J. Ande 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 French ⚖ 907 KB

## Abstract Infection of cervical keratinocytes by high‐risk HPV is involved in the etiology of cervical carcinoma. Since viral products are immunogenic, development of cancer may require suppression of immune responses directed against infected epithelial cells. Many markers of host immune effecto

Antibodies against oncoproteins E6 and E
Antibodies against oncoproteins E6 and E7 of human papillomavirus types 16 and 18 in patients with head-and-neck squamous-cell carcinoma
✍ Klaus Zumbach; Markus Hoffmann; Tomas Kahn; Franz Bosch; Stefan Gottschlich; Tib 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 69 KB 👁 1 views

Human papillomaviruses (HPVs) have been recognized as an essential pathogenic factor in anogenital cancer. HPV DNA has also been found in a subgroup of head-and-neck squamous-cell carcinomas (HNSCCs), and a causative role of the virus in the development of these tumors has been suggested by the conc

Genetic diversity of human papillomaviru
Genetic diversity of human papillomavirus type 16 E6, E7, and L1 genes in Italian women with different grades of cervical lesions
✍ Valeria Cento; Massimo Ciccozzi; Luigi Ronga; Carlo Federico Perno; Marco Ciotti 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 154 KB 👁 1 views

## Abstract High‐risk human papillomaviruses (HPVs) are risk factors for the development of cervical cancer. HPV 16 is the most common type, being present in about 60% of cervical cancers worldwide. Previous studies have reported upon the association between HPV 16 E6 variants and increased risk of

Human papillomavirus (HPV) 16 E6 sensiti
Human papillomavirus (HPV) 16 E6 sensitizes cells to atractyloside-induced apoptosis: Role of p53, ICE-like proteases and the mitochondrial permeability transition
✍ Jacqueline Brown; Hiromi Higo; Ann Mckalip; Brian Herman 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 128 KB

Infection of cervical epithelial cells with certain high risk HPV genotypes is thought to play an etiologic role in the development of cervical cancer. In particular, HPV type 16 and 18 early protein 6 (E6) is thought to contribute to epithelial transformation by binding to the tumor suppressor prot