✦ LIBER ✦
Small zone gel chromatography of interacting systems: Theoretical and experimental evaluation of elution profiles for kinetically controlled macromolecule-ligand reactions
✍ Scribed by John R. Cann; Eunice J. York; John M. Stewart; Juan C. Vera; Ricardo B. Maccioni
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 971 KB
- Volume
- 175
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A phenomenological theory of small zone gel chromatography is elaborated for kinetically controlled macromolecule-ligand interactions. Chromatography, direct binding experiments, and rate measurements are used for successful comparison of experimental behavior with theoretical elution profiles for the interaction of MAP-2 with two synthetic peptide fragments from the C-terminal moieties of alpha- and beta-tubulin subunits. The results of this study provide guidelines for interpretation of experimental small zone elution profiles of total ligand.