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Small stable RNA ofNeurospora crassa

โœ Scribed by Kenneth T. Kim; David Apirion; Basanta K. Ghora

Book ID
104698097
Publisher
Springer
Year
1978
Tongue
English
Weight
685 KB
Volume
160
Category
Article
ISSN
1617-4615

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The ssrA gene, coding for the metabolically stable 10Sa RNA, affects cell growth. A mutant in which the chromosomal 10Sa RNA gene is interrupted by a cat insert does not produce detectable levels of 10Sa RNA, and it grows more slowly than the parental strain.

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Radioactively labeled 4.5S, 6S, and 10S RNAs from Escherichia coli were hybridized to EcoRI fragments from the E. coli genome. Each of these molecules bound to more than one DNA fragment. Cot curve analysis of the kinetics of the annealing of these RNAs to denatured E. coli DNA suggests that the DNA