Small-angle x-ray scattering studies of fibrin film: Comparisons of fine and coarse films prepared with thrombin and ancrod

✍ Scribed by Michael F. Müller; John D. Ferry; J. S. Lin

• Publisher: Wiley (John Wiley & Sons)
• Year: 1989
• Tongue: English
• Weight: 429 KB
• Volume: 28
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360280508

✦ Synopsis

Measurements of small-angle x-ray scattering have been made on films prepared from fine and coarae (i.e., formed at high and low, respectively, pH and ionic strength) clots of bovine fibrin by osmotic ahrinkage or compression in one dimension. Intensity profiles were obtained with pinhole geometry on f i l m s stretched up to a stretch ratio of 1.43. In unstretched coarse films, repeat spacinge were seen at about 245, 120, and 77-80 A. These peaks can probakly be identified with the first, second, and third orders of the well-known fibrin repeat of 225 A. In unstretched fine films, only the 77-80 A spacing w a ~ seen. In this case, the first two orders may be weak because the half-staggered arrangement of monomer units giving rise to the 225 A reflection is not reinforced by lateral aggregation of protofibrils; the third order may be strong since the ~olecular subdomains appear to divide the repeat roughly into thirds. After stretching, the 77-80 A spacing persisted in the rneridional direction but almost disappeared in the equatorial. Experiments on unstretched films prepared with ancrod substituted for thrombin gave similar results.