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Slow magic-angle coil spinning: A high-sensitivity and high-resolution NMR strategy for microscopic biological specimens

✍ Scribed by Alan Wong; Pedro M. Aguiar; Dimitris Sakellariou

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
215 KB
Volume
63
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

High‐resolution magic‐angle spinning has become a valuable tool to study biologic samples; however, there are only a few high‐resolution magic‐angle spinning methodologies that have been explored specifically to deal with small semisolid biomaterials such as tissues. Here, we report a novel high‐resolution ^1^H NMR spectroscopic approach using magic‐angle coil spinning. We demonstrate its potential for intact tissues by combining a resonant microcoil with a large‐volume commercial magic‐angle spinning rotor, where the microcoil offers incredible sensitivity and the large‐volume magic‐angle spinning rotor provides stable slow magic‐angle spinning. This approach provides high‐resolution spectra with high sensitivity and the preservation of living biologic specimens for an accurate chemical analysis. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.

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