SLOW INDUCTION OF GELATINASE B mRNA BY ACIDIC CULTURE CONDITIONS IN MOUSE METASTATIC MELANOMA CELLS
✍ Scribed by YASUMASA KATO; SATORU OZONO; TARO SHUIN; KAORU MIYAZAKI
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 99 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
Abstract
Gelatinase B has been thought to be a key enzyme for degradation of extracellular matrix in tumour invasion and metastasis. In this study, we examined the effect of acidic culture medium (pH5.9) on the expression of gelatinase B mRNA in mouse metastatic melanoma cell line (B16‐F10). Using reverse transcription‐polymerase chain reaction (RT‐PCR) analysis, we found that gelatinase B was induced by the acidic culture medium at 24h, and then gradually diminished to 72h. By gelatin zymographic analysis, gelatinase B was first detected at 24h, continued to increase and then reached a plateau at around 48h. These results suggest that the induction of gelatinase B secretion by acidic culture medium occurs as a result of the gene expression.