H2O2 as a DNA fragmenting agent in the a
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Leszek Szmigiero; Kazimierz Studzian
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Article
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1988
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Elsevier Science
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English
⚖ 423 KB
A method for DNA fragmentation by H2O2 in the DNA alkaline elution procedure is described. Treatment of cell suspensions for 1 h with 100 microM H2O2 or 5 mM H2O2 at 0-1 degree C resulted in DNA breakage equivalent to doses of 300 and 3000 rad of gamma-rays, respectively. The elution profiles were r