Size-Exclusion Chromatography with On-Line Light-Scattering, Absorbance, and Refractive Index Detectors for Studying Proteins and Their Interactions
✍ Scribed by Jie Wen; Tsutomu Arakawa; John S. Philo
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 249 KB
- Volume
- 240
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
small and large molecules in a highly specific manner LS/UV/RI, size-exclusion chromatography with on-line light-scatand hence knowledge of how they interact with other tering, uv absorbance, refractive index detectors; (LS), intensity of molecules is fundamental for understanding their funclight-scattering signal; (UV), intensity of uv absorbance signal; tions. In addition, protein molecules often self-associ-(RI), intensity of refractive index signal; BSA, bovine serum albu- min; RNase, ribonuclease; CHO, Chinese hamster ovary; SCF, ate to oligomers for specific purposes. For newly cloned stem cell factor; bFGF, basic fibroblast growth factor; TNF, tumor proteins, especially those being found by large-scale necrosis factor; sTNFR, soluble TNF type I receptor; BDNF, brainsequencing projects, we often have no idea whether the derived neurotrophic factor; NT-3, neurotrophin-3; mAb, mono- protein exists in solution as a monomer, dimer, or other clonal antibody; HMWH, high-molecular-weight heparin; PBS, phosphate-buffered saline.
oligomer. Therefore, determining the molecular 155