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Site-specific recombination in human embryonic stem cells induced by cell-permeant Cre recombinase

✍ Scribed by Nolden, Lars; Edenhofer, Frank; Haupt, Simone; Koch, Philipp; Wunderlich, F Thomas; Siemen, Henrike; Brüstle, Oliver

Book ID: 109937975
Publisher: Nature Publishing Group
Year: 2006
Tongue: English
Weight: 695 KB
Volume: 3
Category: Article
ISSN: 1552-4450
DOI: 10.1038/nmeth884

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## Abstract Following gene targeting, a __loxP__‐neo‐__loxP__ cassette was introduced into ES cells. The presence of a selectable marker such as neo in the targeted allele may result in gene interference in flox mice or unexpected phenotypes due to genetic ambiguity in direct knockout mice. Typical