Site-specific protein backbone and side-chain NMR chemical shift and relaxation analysis of human vinexin SH3 domain using a genetically encoded 15N/19F-labeled unnatural amino acid

Site-specific 19F NMR chemical shift and

Site-specific 19F NMR chemical shift and side chain relaxation analysis of a membrane protein labeled with an unnatural amino acid

✍ Pan Shi; Hu Wang; Zhaoyong Xi; Chaowei Shi; Ying Xiong; Changlin Tian 📂 Article 📅 2010 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 373 KB

## Abstract Site‐specific ^19^F chemical shift and side chain relaxation analysis can be applied on large size proteins. Here, one‐dimensional ^19^F spectra and __T__~1~, __T__~2~ relaxation data were acquired on a SH3 domain in aqueous buffer containing 60% glycerol, and a nine‐transmembrane helic