Site-Specific Patterning of Biomolecules and Quantum Dots on Functionalized Surfaces Generated by Plasma-Enhanced Chemical Vapor Deposition
✍ Scribed by J. M. Slocik; E. R. Beckel; H. Jiang; J. O. Enlow; J. S. Zabinski Jr.; T. J. Bunning; R. R. Naik
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 457 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0935-9648
No coin nor oath required. For personal study only.
✦ Synopsis
Micropatterned surfaces have received extensive attention for possible applications in advanced technologies including microelectronics, [1,2] microfluidics, [3,4] cell-growth confinement, [5,6] and biosensor fabrication. [7,8] The latter two applications exemplify the increasing coordination between materials science and biology for future-generation advanced materials. Plasma-enhanced chemical vapor deposition (PECVD) shows great promise for strengthening this aforementioned materials science and biology intersection. PECVD provides an excellent generalized platform for the incorporation of a wealth of different biomolecules and/or inorganic materials by way of micropatterned structures. [9] Micropatterned substrates with site-specific binding were developed by way of self-assembled monolayer (SAM) chemistry in conjunction with thin-layer organic polymer deposition via PECVD (Fig. 1). Spatial binding of biomolecules and quantum dots to PECVD-patterned substrates is demonstrated.
Currently, surface patterning of biomolecules involves techniques such as dip-pen nanolithography, [10][11][12] electron- [13] and ion-beam lithography, [14,15] polydimethylsiloxane stamping, [8,16] ink-jet printing, [17] nanoparticle self-assembly, [18] chemical selectivity on patterned gold, [19] and atomic force microscopy (AFM) mediated nanografting. [20,21] These techniques encounter inherent limitations and drawbacks for biomolecular patterning. For instance, electron-beam (e-beam) patterning, nanoparticle self-assembly, and chemical selectivity on patterned gold require multiple steps for pattern formation, which greatly increases the risk of error in the fabrication process. Moreover, e-beam patterning necessitates the use of harsh high-vacuum environments. Nanoparticle self-assembly and chemical selectivity on patterned gold are only capable of single-molecule binding as these techniques make use of the protein resistance of poly(ethylene glycol) to limit the molecular binding to specific chemically compatible areas. AFM-mediated nanografting shows promise for nanometer-
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