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Site-Specific Mutations in the TraI Relaxase and Upstream Region of Plasmid RP4

✍ Scribed by S.P. Cole; D.G. Guiney


Book ID
112263250
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
61 KB
Volume
34
Category
Article
ISSN
0147-619X

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The broad jost range plasmid, RK2, has been isolated as a DNA-protein relaxation complex. Nicking of the plasmid DNA in the relaxation complex occurs at a single specific site (rlx) located approximately 20 kb away from the origin of DNA replication. A cis-acting function required for plasmid transf