✦ LIBER ✦
Site-specific modification of enzymatically synthesized RNA: Transcription initiation and Diels-Alder reaction
✍ Scribed by Burckhard Seelig; Andres Jäschke
- Book ID
- 104258236
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- French
- Weight
- 257 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
✦ Synopsis
Initiator nucleotides consisting of an anthracene ring system coupled to the 5'-phosphate of guanosine monophosphate via a polyethylene glycol (PEG) linker were chemically synthesized. When added to transcription reactions, these initiators were site-specifically incorporated into RNA transcripts yielding RNA conjugates with a fluorophore selectively attached to the 5'-end via a flexible linker. Besides sensitive fluorescence detection, the anthracene allowed the convenient modification with maleimides by Diels-Alder reaction.