✦ LIBER ✦

Site-specific integration of an F′ lac pro factor in the region of the replication origin (oriC) of E. coli

✍ Scribed by LaVerne, Laurie Simon ;Ray, Dan S.

Publisher: Springer
Year: 1980
Tongue: English
Weight: 922 KB
Volume: 179
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425474

No coin nor oath required. For personal study only.

✦ Synopsis

An episome, F128, which carries approximately 8 x 10(4) base pairs of chromosomal DNA homologous to the lac pro region of the E. coli chromosome, has been found to integrate into the oriC region of the chromosome in a site specific reaction. While the event appears to be recA-dependent, no homology between the episome and this region of the chromosome was detected. The Hfr strains formed result from the integration of intact F128 molecules. The structure of the Hfr strains generated has been determined and their transfer properties analyzed.

📜 SIMILAR VOLUMES

Origin of replication, oriC, of the Esch

Origin of replication, oriC, of the Escherichia coli chromosome: Mapping of genes relative to R.EcoRI cleavage sites in the oriC region

✍ von Meyenburg, Kaspar ;Hansen, Flemming G. ;Nielsen, Lissie D. ;Jørgensen, Poul 📂 Article 📅 1977 🏛 Springer 🌐 English ⚖ 820 KB

A precise genetic-physical map of the tnailv region at 82 rain on the genetic map of E. coli is obtained through deletion mapping and analysis by restriction endonuclease EcoRI of plasmids, derived from an F ~ carrying the genes between aroE and ilv. A locus, designated het, which in its diploid st