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Site-Specific Incorporation of Tryptophan Analogues into Recombinant Proteins in Bacterial Cells

✍ Scribed by Kwon, Inchan; Tirrell, David A.

Book ID
126184047
Publisher
American Chemical Society
Year
2007
Tongue
English
Weight
345 KB
Volume
129
Category
Article
ISSN
0002-7863

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Three different methods to quantitate tryptophan (Trp) analogue incorporation into recombinant proteins are described: first, spectroscopic analysis based on a linear combination of the absorption spectra of the aromatic residues in the denatured Trp-containing or analogue-substituted protein; secon